Immunofluorescence:Article Title: Efficient pulmonary lymphatic drainage is necessary for inflammation resolution in ARDS
Article Snippet: .. Immunofluorescence staining on sections was performed using primary antibodies against Lyve-1 (rabbit, 1:500; Abcam, catalog ab14917), VEGFR-3 (goat, 1:500, R&D Systems, AF743), VE-cadherin (rabbit, 1:500; Abcam, catalog ab205336), CCL21 (rabbit, 1:500; Abcam, catalog ab231116), F4/80 (rabbit, 1:100; Abcam, catalog ab16911), LY6G (rat, 1:100; Abcam, catalog ab210204), CD68 (rat, 1:200; Abcam, catalog ab237968), CD31 (rabbit, 1:100; Abcam, catalog ab222783), and Ki-67 (rabbit, 1:200; Thermo Fisher Scientific, catalog PA5-19462), or TUNEL-FITC (Abcam, catalog ab66108). .. The corresponding secondary antibodies were used: goat anti-rabbit DyLight 594 (1:200, Invitrogen, catalog 35560), goat anti-rabbit Alexa Fluor 488 (1:200, Abcam, catalog ab150077), donkey anti-goat Alexa Fluor 488 (1:200, Invitrogen, catalog A32814), donkey anti-goat Alexa Fluor 594 (1:200, Abcam, catalog ab 150132), and goat anti-rat Alexa Fluor 488 (1:500, Cell Signaling Technology, catalog 4416S/4417S).
Staining:Article Title: Efficient pulmonary lymphatic drainage is necessary for inflammation resolution in ARDS
Article Snippet: .. Immunofluorescence staining on sections was performed using primary antibodies against Lyve-1 (rabbit, 1:500; Abcam, catalog ab14917), VEGFR-3 (goat, 1:500, R&D Systems, AF743), VE-cadherin (rabbit, 1:500; Abcam, catalog ab205336), CCL21 (rabbit, 1:500; Abcam, catalog ab231116), F4/80 (rabbit, 1:100; Abcam, catalog ab16911), LY6G (rat, 1:100; Abcam, catalog ab210204), CD68 (rat, 1:200; Abcam, catalog ab237968), CD31 (rabbit, 1:100; Abcam, catalog ab222783), and Ki-67 (rabbit, 1:200; Thermo Fisher Scientific, catalog PA5-19462), or TUNEL-FITC (Abcam, catalog ab66108). .. The corresponding secondary antibodies were used: goat anti-rabbit DyLight 594 (1:200, Invitrogen, catalog 35560), goat anti-rabbit Alexa Fluor 488 (1:200, Abcam, catalog ab150077), donkey anti-goat Alexa Fluor 488 (1:200, Invitrogen, catalog A32814), donkey anti-goat Alexa Fluor 594 (1:200, Abcam, catalog ab 150132), and goat anti-rat Alexa Fluor 488 (1:500, Cell Signaling Technology, catalog 4416S/4417S).
TUNEL Assay:Article Title: Efficient pulmonary lymphatic drainage is necessary for inflammation resolution in ARDS
Article Snippet: .. Immunofluorescence staining on sections was performed using primary antibodies against Lyve-1 (rabbit, 1:500; Abcam, catalog ab14917), VEGFR-3 (goat, 1:500, R&D Systems, AF743), VE-cadherin (rabbit, 1:500; Abcam, catalog ab205336), CCL21 (rabbit, 1:500; Abcam, catalog ab231116), F4/80 (rabbit, 1:100; Abcam, catalog ab16911), LY6G (rat, 1:100; Abcam, catalog ab210204), CD68 (rat, 1:200; Abcam, catalog ab237968), CD31 (rabbit, 1:100; Abcam, catalog ab222783), and Ki-67 (rabbit, 1:200; Thermo Fisher Scientific, catalog PA5-19462), or TUNEL-FITC (Abcam, catalog ab66108). .. The corresponding secondary antibodies were used: goat anti-rabbit DyLight 594 (1:200, Invitrogen, catalog 35560), goat anti-rabbit Alexa Fluor 488 (1:200, Abcam, catalog ab150077), donkey anti-goat Alexa Fluor 488 (1:200, Invitrogen, catalog A32814), donkey anti-goat Alexa Fluor 594 (1:200, Abcam, catalog ab 150132), and goat anti-rat Alexa Fluor 488 (1:500, Cell Signaling Technology, catalog 4416S/4417S).
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